Optimized protocol for whole organ decellularization
نویسندگان
چکیده
منابع مشابه
Optimized protocol for whole organ decellularization
BACKGROUND The idea of tissue decellularization to gain matrices for tissue engineering is promising. The aim of the present study is to establish a safe and reproducible protocol for solid tissue decellularization that prevents the architecture of the matrix with the inherent vascular network. METHODS The study was performed in rat kidneys which were decellularized by a SDS-based perfusion p...
متن کاملThe use of whole organ decellularization for the generation of a vascularized liver organoid.
UNLABELLED A major roadblock to successful organ bioengineering is the need for a functional vascular network within the engineered tissue. Here, we describe the fabrication of three-dimensional, naturally derived scaffolds with an intact vascular tree. Livers from different species were perfused with detergent to selectively remove the cellular components of the tissue while preserving the ext...
متن کاملOptimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering
Aim. To refine the decellularization protocol of whole porcine liver, which holds great promise for liver tissue engineering. Methods. Three decellularization methods for porcine livers (1% sodium dodecyl sulfate (SDS), 1% Triton X-100 + 1% sodium dodecyl sulfate, and 1% sodium deoxycholate + 1% sodium dodecyl sulfate) were studied. The obtained liver scaffolds were processed for histology, res...
متن کاملDecellularization and recellularization of whole livers.
The liver is a complex organ which requires constant perfusion for delivery of nutrients and oxygen and removal of waste in order to survive. Efforts to recreate or mimic the liver microstructure with grounds up approach using tissue engineering and microfabrication techniques have not been successful so far due to this design challenge. In addition, synthetic biomaterials used to create scaffo...
متن کاملOptimization of SDS exposure on preservation of ECM characteristics in whole organ decellularization of rat kidneys.
Renal transplantation is well established as the optimal form of renal replacement therapy but is restricted by the limited pool of organs available for transplantation. The whole organ decellularisation approach is leading the way for a regenerative medicine solution towards bioengineered organ replacements. However, systematic preoptimization of both decellularization and recellularization pa...
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ژورنال
عنوان ژورنال: European Journal of Medical Research
سال: 2017
ISSN: 2047-783X
DOI: 10.1186/s40001-017-0272-y